Changes in circulating microRNA levels associated with prostate cancer
Open Access
- 12 January 2012
- journal article
- research article
- Published by Springer Nature in British Journal of Cancer
- Vol. 106 (4), 768-774
- https://doi.org/10.1038/bjc.2011.595
Abstract
The aim of this study was to investigate the hypothesis that changes in circulating microRNAs (miRs) represent potentially useful biomarkers for the diagnosis, staging and prediction of outcome in prostate cancer. Real-time polymerase chain reaction analysis of 742 miRs was performed using plasma-derived circulating microvesicles of 78 prostate cancer patients and 28 normal control individuals to identify differentially quantified miRs. A total of 12 miRs were differentially quantified in prostate cancer patients compared with controls, including 9 in patients without metastases. In all, 11 miRs were present in significantly greater amounts in prostate cancer patients with metastases compared with those without metastases. The association of miR-141 and miR-375 with metastatic prostate cancer was confirmed using serum-derived exosomes and microvesicles in a separate cohort of patients with recurrent or non-recurrent disease following radical prostatectomy. An analysis of five selected miRs in urine samples found that miR-107 and miR-574-3p were quantified at significantly higher concentrations in the urine of men with prostate cancer compared with controls. These observations suggest that changes in miR concentration in prostate cancer patients may be identified by analysing various body fluids. Moreover, circulating miRs may be used to diagnose and stage prostate cancer.Keywords
This publication has 17 references indexed in Scilit:
- MicroRNA in Prostate, Bladder, and Kidney Cancer: A Systematic ReviewEuropean Urology, 2011
- Circulating miRNAs are correlated with tumor progression in prostate cancerInternational Journal of Cancer, 2010
- Gene Networks and microRNAs Implicated in Aggressive Prostate CancerCancer Research, 2009
- Distinct MicroRNA Alterations Characterize High- and Low-Grade Bladder CancerCancer Research, 2009
- Causes and consequences of microRNA dysregulation in cancerNature Reviews Genetics, 2009
- MicroRNAs: Target Recognition and Regulatory FunctionsCell, 2009
- CMA – a comprehensive Bioconductor package for supervised classification with high dimensional dataBMC Bioinformatics, 2008
- Circulating microRNAs as stable blood-based markers for cancer detectionProceedings of the National Academy of Sciences, 2008
- Active surveillance for early‐stage prostate cancerCancer, 2008
- The Influence of Finasteride on the Development of Prostate CancerNew England Journal of Medicine, 2003