Biomethylation of deoxyribonucleic acid in cultured human tumour cells (HeLa). Methylated bases other than 5-methylcytosine not detected
- 1 June 1972
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 128 (1), 133-138
- https://doi.org/10.1042/bj1280133
Abstract
1. Incorporation of methyl groups from [methyl-14C]methionine into DNA of dividing HeLa cells was investigated, essentially by the procedures of Culp, et al. (1970). 2. Contrary to the report of the latter, but in agreement with other work on biomethylation of mammalian DNA, 5-methylcytosine was the sole methylated base detected. 3. Chromatographic separations of 3-methylcytosine from 5-methylcytosine and purines are discussed.Keywords
This publication has 12 references indexed in Scilit:
- Methylation of ribonucleic acid by the carcinogens dimethyl sulphate, N-methyl-N-nitrosourea and N-methyl-N′-nitro-N-nitrosoguanidine. Comparisons of chemical analyses at the nucleoside and base levelsBiochemical Journal, 1972
- Methylation of deoxyribonucleic acid in cultured mammalian cells by N-methyl-N′-nitro-N-nitrosoguanidine. The influence of cellular thiol concentrations on the extent of methylation and the 6-oxygen atom of guanine as a site of methylationBiochemical Journal, 1970
- Methylated bases in DNA of animal originArchives of Biochemistry and Biophysics, 1970
- Rapid separation of nucleosides and nucleotides by cation-exchange column chromatographyJournal of Chromatography A, 1969
- Possible Relevance of O–6 Alkylation of Deoxyguanosine to the Mutagenicity and Carcinogenicity of Nitrosamines and NitrosamidesNature, 1969
- Ribonucleic Acid Polymerase Reactions with Methylated Polycytidylic Acid TemplatesJournal of Biological Chemistry, 1968
- Isolation of deoxyribonucleic acid from mammalian tissuesBiochemical Journal, 1967
- Carcinogenic Nitroso CompoundsAdvances in Cancer Research, 1967
- Effects of Some Chemical Mutagens and Carcinogens on Nucleic AcidsProgress in Nucleic Acid Research and Molecular Biology, 1966
- Methylation of nucleic acids in Hela cellsBiochemical and Biophysical Research Communications, 1965