Messenger ribonucleic acids from pig intestinal mucosa direct synthesis of calcium-binding protein in a cell-free translation system

Abstract

Messenger RNA from pig duodenal mucosa directs synthesis in a wheat-germ cell-free system of 2 products precipitated by antiserum to pure Ca binding protein. One of these products has a higher MW than authentic Ca binding protein, but, like the authentic protein, is heat-stable. The other protein co-migrates with pure Ca-binding protein on sodium dodecyl sulfate/polyacrylamide-gel electrophoresis, is stable on heating to 70.degree. C for 15 min and alters its elution position on ion-exchange chromatography depending on whether Ca2+ is present in or absent from the elution buffer. The synthesized protein has these properties in common with authenic Ca-binding protein.