Chronic exposure to glibenclamide impairs insulin secretion in isolated rat pancreatic islets

Abstract

We investigated the effect of 24 h exposure to 100 nmol/l glibenclamide on insulin secretion in isolated rat pancreatic islets. The insulin content was similar in control islets and in islets preincubated with 100 nmol/l glibenclamide for 24 h. In islets preexposed to glibenclamide: 1) the subsequent response to a maximal glibenclamide stimulatory concentration (10 µmol/l, 1 h at 37 C) was greatly reduced in comparison to control islets (0.69 ± 0.20 % vs 2.16 ± 0.41%; mean ± SE; n=14; p vs 2.38 ± 0.44 %; n=8; p p