Regulation of Cytoskeletal Protein Organization and Expression in Human Granulosa Cells in Response to Gonadotropin Treatment*
- 1 February 1989
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 124 (2), 1033-1041
- https://doi.org/10.1210/endo-124-2-1033
Abstract
The organization and expression of the major cytoskeletal protein systems and of several cytoskeleton-associated proteins were analyzed in human granulosa cells obtained from preovulatory follicles in the course of an in vitro fertilization program. The cells were cultured in medium containing 5% fetal calf serum in the absence or presence of gonadotropins. Within several hours after the addition of hCG to cultured human granulosa cells, the cells acquired a rounded and aggregated morphology with numerous thin and long processes. Cells cultured without the gonadotropin had a flat and extended morphology on the substrate, with a well developed stress fiber system and numerous large vinculin-containing adhesion plaques. The [35S]methionine-labeled protein pattern of hCG-treated cells obtained by two-dimensional isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a 7- to 10-fold decrease in the synthesis of vinculin and the nonmuscle tropomyosin isoforms, and a 2- to 4-fold decreased in the synthesis of .alpha.-actinin and actin, without a comparable change in .alpha.- and .beta.-tubulin synthesis. In the absence of gonadotropins, human granulosa cells synthesized both the intermediate filament (IF) protein vimentin and cytokeratin-type IF proteins 8 and 18 and, to a lesser extent, cytokeratins 7 and 19. By immunofluoresence, both type of IF networks were detected in untreated cells, while hCG-treated cells synthesized and contained only vimentin-type IF networks. Thus maintenance of the steroidogenic capacity in the presence of gonadotropins in human granulosa-luteal cells involves specific changes in cell shape and in both the organization and the expression of the microfilament and IF systems.This publication has 9 references indexed in Scilit:
- In vitro regulation of granulosa cell differentiation. Involvement of cytoskeletal protein expression.Journal of Biological Chemistry, 1987
- Microtubules and the Gonadotropic Regulation of Granulosa Cell Steroidogenesis*Endocrinology, 1987
- Gonadotropins and cyclic adenosine 3',5'-monophosphate (cAMP) alter the morphology of cultured human granulosa cellsBiology of Reproduction, 1986
- A 90,000-dalton binding protein common to both steroid receptors and the Rous sarcoma virus transforming protein, pp60v-src.Journal of Biological Chemistry, 1985
- Purification and characterization of multiple isoforms of tropomyosin from rat cultured cells.Journal of Biological Chemistry, 1985
- Evidence that the 90-kDa phosphoprotein associated with the untransformed L-cell glucocorticoid receptor is a murine heat shock protein.Journal of Biological Chemistry, 1985
- Isolation and characterization of tropomyosin-containing microfilaments from cultured cells.Journal of Biological Chemistry, 1983
- Unpolymerized tubulin modulates the level of tubulin mRNAsCell, 1981
- Hormonal regulation of cytodifferentiation and intercellular communication in cultured granulosa cells.Proceedings of the National Academy of Sciences, 1981