Studies on diffusing factors

Abstract

Cl. butylicum produces viscous metabolites when grown in a glucose-asparagine-mineral salts medium containing Bacto-peptone as a source of biotin. Both growth and production of viscous metabolites are partially inhibited by inclusion of hyaluronidase concentrates in the medium. There are optimal concs. of glucose and peptone for bacterial growth and for production of viscous materials. The optimal conditions for bacterial growth are not identical with those optimal for the production of viscous metabolites.