Partial Characterization of Glycopeptides from Culture Filtrates of Fulvia fulva (Cooke) Ciferri (syn. Cladosporium fulvum), the Tomato Leaf Mould Pathogen

Abstract

Culture filtrates of races 0, 4 and 1.2.3. of F. fulva were fractionated by sequential gel chromatography on Bio-Gel P-2, ion-exchange chromatography on DEAE-cellulose and affinity chromatography on concanavalin A-Sepharose 4B. The fractions were characterized for their carbohydrate, phosphate and protein contents and sugar compositions. D-Galactose, D-glucose and D-mannose were the major sugars with much smaller amounts of D-glucuronic acid, D-galactosamine and D-glucosamine. Phosphate was present as a diester. The carbohydrate was present in a family of glycopeptides of disperse MW, charge and sugar composition. The molecular organization of these polymers was further investigated by mild acid and alkali degradation and by acetolysis of the polysaccharide remaining after these treatments. A possible structure for the polymers is suggested.