Disc electrophoretic studies of intraspecific variability of proteins from dermatophytes
- 1 January 1968
- journal article
- Published by Oxford University Press (OUP) in Medical Mycology
- Vol. 6 (2), 133-137
- https://doi.org/10.1080/00362176885190241
Abstract
Four different human isolates of Microsporum canis, Trichophyton mentagrophytes, T. rubrum, T. tonsurans and Epidermophyton floccosum were cultured for 14 days in Sabouraud's medium at room temperature. The non-dialyzable proteins of the culture filtrates were fractionated by disc electrophoresis. The number and electrophoretic mobility of the protein fractions of the 4 isolates of each species were very similar. Interspecific electrophoretic differences, however, werepresent. Some variations in optical densities of homologous fractions were observed within each species. These data suggest that the qualitative electrophoretic patterns of these species are relatively stable taxonomic characteristics. On cultive pendant 14 jours sur milieu de Sabouraud à la température de laboratoire 4 souches differéntes de Microsporum canis, de Trichophyton mentagrophytes, de T. rubrum, de T. tonsurans et d'Epidermophyton floccosum, toutes d'origine humaine. Les protéines non dialysables des filtrats de culture sont fractionnées par électrophorèse sur gel d'acrylamide. Le nombre et la mobilité électrophorétique des fractions protéiniques des quatre souches de chaque espèce sont très semblables. Cependant des différences életrophorétiques interspécifiques se sont manifestées. A l'intérieur de chaque espèce, on observe des variations dans les densités optiques des fractions homologues. Ces résultats suggèrent que du point de vue qualitatif les électrophorégrammes de ces espèces sont des caractères taxinomiques relativement stables.Keywords
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