Human immune interferon: induction in lymphoid cells by a calcium ionophore

Abstract
Human lymphocyte cultures produced interferon after treatment with the calcium ionophore A23187, but not after treatment with potassium ionophore nonactin. Interferon production was detectable between 3-6 h after ionophore treatment and reached the maximum level between 12-24 h. Ionophore-induced interferon appeared to be immune interferon on the basis of acid lability, lack of neutralization by antibody to leukocyte interferon, slow kinetics of activation of the antiviral state and molecular characterization by isoelectrofocusing.