Vacuum Freeze Drying of Frozen Sections for Dry-Mounting, High-Resolution Autoradiography
- 1 January 1964
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 39 (4), 219-223
- https://doi.org/10.3109/10520296409061233
Abstract
Specimens no larger than 1.5 × 1.5 × 2 mm were frozen in liquid nitrogen and sectioned, while still frozen, with a refrigerated microtome. The frozen sections were dried in a vacuum, then pressed onto either Kodak NTB10 plates or onto slides which had been coated with Kodak NTB3 emulsion and dried. Radioactive mouse liver was used to test tissue preservation. Intestinal mucosa with Ha-labeled nuclei was used to test the quality of autoradiography. Good cytological detail was preserved in both tissues, with the autoradiographs interpretable at the cellular level.Keywords
This publication has 4 references indexed in Scilit:
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- Dry, High Resolution AutoradiographyStain Technology, 1962
- DRY - MOUNTING AUTORADIOGRAPHIC TECHNIC FOR INTRACELLULAR LOCALIZATION OF WATER-SOLUBLE COMPOUNDS IN TISSUE SECTIONS1961
- The application of the freezing‐drying technique to retinal histochemistryJournal of Cellular and Comparative Physiology, 1942