Techniques to Measure DNA Single-strand Breaks in Cells: A Review
- 1 January 1988
- journal article
- review article
- Published by Taylor & Francis in International Journal of Radiation Biology
- Vol. 54 (5), 695-707
- https://doi.org/10.1080/09553008814552151
Abstract
Alkaline sucrose sedimentation was for a number of years the standard procedure for the measurement of single-strand breaks. Some years ago a number of new techniques with improved sensitivity were introduced. The following techniques are presented and discussed: alkaline unwinding, alkaline filter elution, nucleoid sedimentation, viscoelastrometry, microelectrophoresis of single cells, DNA precipitation, pulse field gel electrophoresis, fluctuation spectroscopy and nick translation.Keywords
This publication has 33 references indexed in Scilit:
- Electrophoretic Separations of Large DNA Molecules by Periodic Inversion of the Electric FieldScience, 1986
- DNA repair kinetics after low doses of X-raysMutation Research/DNA Repair Reports, 1984
- X-ray induced DNA double strand break production and repair in mammalian cells as measured by neutral filter elutionNucleic Acids Research, 1979
- Spectrofluorometric Measurement of the Binding of Ethidium to Superhelical DNA from Cell NucleiEuropean Journal of Biochemistry, 1978
- REPAIR OF DNA BREAKS INDUCED BY GAMMA RAYS AND FAST NEUTRONS IN CHINESE HAMSTER CELLSPublished by Elsevier ,1978
- Supercoils in human dnaJournal of Cell Science, 1975
- Two Forms of Repair of DNA in Mammalian Cells following IrradiationBiophysical Journal, 1970
- The rate of strand separation in alkali-treated DNAJournal of Molecular Biology, 1966
- Interruptions in the polynucleotide strands in bacteriophage DNAJournal of Molecular Biology, 1964
- Sedimentation Rate as a Measure of Molecular Weight of DNABiophysical Journal, 1963