The (Na+,K+)ATPase exhibits enzymic activity in the absence of the glycoprotein subunit
- 8 August 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 159 (1-2), 280-284
- https://doi.org/10.1016/0014-5793(83)80464-5
Abstract
Membrane-bound (Na+,K+)ATPase from avian nasal salt glands was exposed to limited papain digestion. Such treatment results in the selective removal of the β-subunit rendering the α-subunit still membrane-bound and expressing full enzymic activity. With further exposure to papain the α-chain becomes fragmented into two major polypeptide components. The fragmented membrane-bound catalytic chain is extremely sensitive to detergent treatment and cannot be solubilized in an active state.Keywords
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