An HPLC Detection System for Phenylurea Herbicides Using Post-Column Photolysis and Chemical Derivatization
- 1 November 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 23 (11), 516-520
- https://doi.org/10.1093/chromsci/23.11.516
Abstract
A procedure is presented for the HPLC separation and detection of phenylurea herbicides. The separated compounds are photochemically degraded, reacted with orthophthalaldehyde- mercaptoethanol (OPA-MERC), and measured fluorometrically. The response of 14 individual herbicide standards to the detection system is reported. Those herbicides with an N,N-dimethyl group give a greater response than those with an N-methyl, N-methoxy moiety. This system is applicable to isocratic and gradient LC and offers much better selectivity than UV detection systems.This publication has 2 references indexed in Scilit:
- Dynamic Fluorogenic Labelling of Pesticides for High Performance Liquid Chromatography: Detection of N-Methylcarbamates with o-PhthalaldehydeAnalytical Letters, 1977
- Evaluation and Confirmation of an Alkylation-Gas-Liquid Chromatographic Method for the Determination of Carbamate and Urea Herbicides in FoodsJournal of AOAC INTERNATIONAL, 1976