Identification of the interferon regulatory factor 5 gene (IRF-5) as a direct target for p53
- 25 April 2002
- journal article
- research article
- Published by Springer Nature in Oncogene
- Vol. 21 (18), 2914-2918
- https://doi.org/10.1038/sj.onc.1205459
Abstract
Interferon regulatory factors (IRFs) regulate transcription of interferon genes through DNA sequence-specific binding to these targets. Using a differential display method for examining gene expression in p53-defective cells infected with adenovirus containing wild-type p53, we found that expression of interferon regulatory factor 5 (IRF-5) mRNA was increased in the presence of exogenous p53. An electrophoretic mobility-shift assay showed that a potential p53 binding site (p53BS) detected in exon 2 of the IRF-5 gene could in fact bind to p53 protein. Moreover, a heterologous reporter assay revealed that the p53BS possessed p53-dependent transcriptional activity. Expression of IRF-5 was induced in p53+/+ cells (MCF7 and NHDF), but not inp53−/− cells (H1299) when DNA was damaged by γ-irradiation, UV-radiation, or adriamycin treatment in a wild-type p53-dependent manner. These results suggest that IRF-5 is a novel p53-target, and that it might mediate the p53-dependent immune response.Keywords
This publication has 21 references indexed in Scilit:
- p53DINP1, a p53-Inducible Gene, Regulates p53-Dependent ApoptosisMolecular Cell, 2001
- Cooperation of the tumour suppressors IRF-1 and p53 in response to DNA damageNature, 1996
- An IRF-1-dependent pathway of DNA damage-induced apoptosis in mitogen-activated T lymphocytesNature, 1995
- Molecular cloning ofLSIRF, a lymphoid-specific member of the interferon regulatory factor family that binds the interferon-stimulated response element (ISRE)Nucleic Acids Research, 1995
- Tumor suppressor p53 is a direct transcriptional activator of the human bax geneCell, 1995
- Cellular commitment to oncogene-induced transformation or apoptosis is dependent on the transcription factor IRF-1Cell, 1994
- p53 tagged sites from human genomic DNAHuman Molecular Genetics, 1994
- The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinasesCell, 1993
- WAF1, a potential mediator of p53 tumor suppressionCell, 1993
- Definition of a consensus binding site for p53Nature Genetics, 1992