FINE STRUCTURAL ALTERATIONS OF INTERPHASE NUCLEI OF LYMPHOCYTES STIMULATED TO GROWTH ACTIVITY IN VITRO
Open Access
- 1 December 1968
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 39 (3), 630-660
- https://doi.org/10.1083/jcb.39.3.630
Abstract
This report describes fine structural changes of interphase nuclei of human peripheral blood lymphocytes stimulated to growth by short-term culture with phytohemagglutinin. Chromatin is found highly labile, its changes accompanying the sequential increases of RNA and DNA synthesis which are known to occur in lymphocyte cultures. In "resting" lymphocytes, abundant condensed chromatin appears as a network of large and small aggregates. Early in the response to phytohemagglutinin, small aggregates disappear during increase of diffuse chromatin regions. Small aggregates soon reappear, probably resulting from disaggregation of large masses of condensed chromatin. Loosened and highly dispersed forms then appear prior to the formation of prophase chromosomes. The loosened state is found by radioautography to be most active in DNA synthesis. Small nucleoli of resting lymphocytes have concentric agranular, fibrillar, and granular zones with small amounts of intranucleolar chromatin. Enlarging interphase nucleoli change chiefly (1) by increase in amount of intranucleolar chromatin and alteration of its state of aggregation and (2) by increase in granular components in close association with fibrillar components.Keywords
This publication has 33 references indexed in Scilit:
- The ultrastructure of the nuclear peripheryJournal of Ultrastructure Research, 1967
- THE STRANDEDNESS OF MEIOTIC CHROMOSOMES FROM ONCOPELTUS The Journal of cell biology, 1966
- Fine structure of interphase liver cell nuclei in subacute ethionine intoxicationExperimental and Molecular Pathology, 1965
- Macromolecular Organization of Nuclei and Chromosomes: A Folded Fibre Model Based on Whole-Mount Electron MicroscopyNature, 1965
- The fine structure of isolated chromosomesJournal of Ultrastructure Research, 1965
- Cytochimie ultrastructurale du nucléoleExperimental Cell Research, 1964
- A CORRELATED LIGHT AND ELECTRON MICROSCOPE STUDY OF THE NUCLEOLAR MATERIAL DURING MITOSIS IN VICIA FABA The Journal of cell biology, 1963
- THE FINE STRUCTURE OF THE DNP COMPONENT OF THE NUCLEUSThe Journal of cell biology, 1963
- The Kinetics of Cell Proliferation in Cultures of Human Peripheral BloodBlood, 1962
- IMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODSThe Journal of cell biology, 1961