Altered lipolytic response to glucagon and adenosine deaminase in adipocytes from starved rats

Abstract

Adipocytes isolated from epididymal adipose tissue of fed or 24 h-starved rats were incubated with a range of glucagon concentrations in the presence and absence of adenosine deaminase (4 munits/ml). With adenosine deaminase present, the lipolytic response to low concentrations of glucagon (1-6 ng/ml) was considerably enhanced in cells from starved rats. The effect of adenosine deaminase on basal lipolysis was altered after starvation. D-3-Hydroxybutyrate (5 mM) decreased the sensitivity of lipolysis to glucagon. The possible involvement of glucagon-stimulated lipolysis in the regulation of ketogenesis is briefly discussed.