A Discontinuous Buffer System for Paper Electrophoresis of Human Hemoglobins

Abstract

A method for paper electrophoresis of hemoglobins in a discontinuous buffer system is presented. Tris-EDTA- borate buffer 0.12M., pH 9.1, is applied to the paper. Barbital buffer 0.06 M., pH 8.6, is used in the buffer vessels. The discontinuous buffer system enables the detection of hemoglobin A₂ in small samples of hemolysate. Resolution of abnormal hemoglobins is superior to that in barbital or Tris-EDTA-borate buffer alone. It offers special advantage for the examination of aged samples of hemoglobin. Reasons for the behavior of the hemoglobins in this buffer system are discussed.