THE INDUCTION OF MELANIZATION IN GOLDFISH SCALES WITH ACTH, IN VITRO
Open Access
- 1 July 1963
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 18 (1), 181-194
- https://doi.org/10.1083/jcb.18.1.181
Abstract
The induction of melanization in xanthic goldfish scales with ACTH in vitro has been studied by light and electron microscopy utilizing ammoniated silver nitrate staining of premelanin and melanin. The melanized cells (melanophores and melanocytes) and the yellow pigmented cells (lipo-phores and the newly described lipocytes) were found to possess many similarities at the levels of cellular and subcellular structure. The latter cells contain characteristic cytoplasmic bodies which react positively to the premelanin stain. Changes accompanying ACTH stimulation of goldfish scales in tissue culture suggest that these bodies in the lipocytes and lipophores can become melanized. Electron micrographs illustrate the intermediate staining of newly formed melanin granules in an induced melanocyte and the appearance of a transitional melanolipophore. It is postulated that ACTH can promote the association of the enzyme tyrosinase with the preformed structure of unmelanized granules..This publication has 15 references indexed in Scilit:
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