Capillary electrophoresis off‐line matrix‐assisted laser desorption/ionisation mass spectrometry of intact and digested proteins using cationic‐coated capillaries
- 4 November 2004
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 18 (23), 2946-2952
- https://doi.org/10.1002/rcm.1712
Abstract
Capillary electrophoresis (CE) was coupled off-line with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS) for the analysis of proteins and peptides. CE fractions were collected directly on a matrix-coated MALDI target, using a sheath-flow interface. Protein adsorption during CE separations was prevented by coating the capillaries with the physically adsorbed, cationic polymer PolyE-323. The CE/MALDI-MS system was used for the analysis of model proteins and peptides at physiological pH as well as analysis of proteins in tear fluid. Moreover, tryptic on-target digestion of the collected protein fractions, with subsequent MALDI-MS and MS/MS peptide analysis, was demonstrated. Copyright © 2004 John Wiley & Sons, Ltd.Keywords
This publication has 28 references indexed in Scilit:
- Capillary electrophoresis using a surfactant-treated capillary coupled with offline matrix-assisted laser desorption ionization mass spectrometry for high efficiency and sensitivity detection of proteinsJournal of Chromatography A, 2001
- Fraction collection in micropreparative capillary zone electrophoresis and capillary isoelectric focusingElectrophoresis, 2000
- Investigation of the Potential of Capillary Electrophoresis with Off-Line Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Clinical Analysis: Examination of a Glycoprotein Factor Associated with Cancer CachexiaAnalytical Chemistry, 1999
- Analysis of recombinant DNA-derived glycoproteins via high-performance capillary electrophoresis coupled with off-line matrix-assisted laser desorption ionization time-of-flight mass spectrometryJournal of Chromatography B: Biomedical Sciences and Applications, 1997
- Coaxial capillary and conductive capillary interfaces for collection of fractions isolated by capillary electrophoresisAnalytical Chemistry, 1995
- Capillary electrophoresis—matrix-assisted laser-desorption ionization mass spectrometry of proteinsJournal of Chromatography A, 1994
- New advanced analytical tools in peptide/protein research: matrix assisted laser desorption mass spectrometryJournal of Controlled Release, 1994
- Matrix-assisted laser desorption mass spectrometry of proteins isolated by capillary zone electrophoresisAnalytical Chemistry, 1992
- Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltonsAnalytical Chemistry, 1988
- Capillary Zone ElectrophoresisScience, 1983