Protein Resistance of PNIPAAm Brushes: Application to Switchable Protein Adsorption
- 18 September 2009
- journal article
- research article
- Published by American Chemical Society (ACS) in Langmuir
- Vol. 26 (3), 1786-1795
- https://doi.org/10.1021/la902505q
Abstract
Protein adsorption, as the primary process occurring when a foreign surface comes into contact with a biosystem, was studied on thin polymer brush films consisting of poly(N-isopropylacrylamide) (PNIPAAm) and poly(2-vinylpyridine) (P2VP). These films were prepared by the “grafting to” method. The protein resistance of stimuli responsive PNIPAAm-brushes toward serum albumin was recorded and compared with protein adsorption on P2VP brushes. To achieve a better understanding of protein resistance, PNIPAAm brushes with different molecular weights were investigated below and above the lower critical solution temperature of 32 °C. To use these findings for the adjustment and switching of protein adsorption, in a first attempt the adsorption on a mixed brush system consisting of PNIPAAm and P2VP chains was studied. This system showed temperature-dependent adsorption behavior due to the presence of PNIPAAm, representing a smart surface with stimuli-responsive changes in the physicochemical surface properties. With this mixed brush, the adsorbed amount of protein could be controlled, depending on composition and the temperature of the surroundings.Keywords
This publication has 54 references indexed in Scilit:
- In-Situ Investigation of the Adsorption of Globular Model Proteins on Stimuli-Responsive Binary Polyelectrolyte BrushesLangmuir, 2006
- Electrokinetic properties of natural fibresColloids and Surfaces A: Physicochemical and Engineering Aspects, 2005
- Electrokinetic Characterization of Poly(Acrylic Acid) and Poly(Ethylene Oxide) Brushes in Aqueous Electrolyte SolutionsLangmuir, 2005
- Gradient Mixed Brushes: “Grafting To” ApproachMacromolecules, 2004
- Interaction of dissolved proteins with spherical polyelectrolyte brushesMacromolecular Symposia, 2003
- Synthesis and swelling behavior of a weak polyacid brushThe Journal of Chemical Physics, 2002
- Phase transition in swollen gels : Part 32. Temperature transition in charged poly(N-isopropylmethacrylamide) hydrogels in water and aqueous NaCl solutionsPhysical Chemistry Chemical Physics, 2002
- Analysis of the Prevention of Protein Adsorption by Steric Repulsion TheoryPublished by American Chemical Society (ACS) ,1995
- Characterization of solid surfaces by electrokinetic measurementsProgress in Organic Coatings, 1989
- Hydrated Dynamic SurfacesAsaio Journal, 1987