Restriction and Modification in Bacillus subtilis. Localization of the Methylated Nucleotide in the BsuRI Recognition Sequence
- 1 October 1978
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 90 (3), 581-583
- https://doi.org/10.1111/j.1432-1033.1978.tb12638.x
Abstract
Calf thymus DNA was methylated in vitro with cell extracts of Bacillus subtilis OG3R (r+m+) and S-adenosyl[Me-3H]methionine. After depurination of the [3H]methylated DNA, the analysis of the pyrimidine dinucleotides revealed the following positions of the methylated nucleosides (indicated by an asterisk) within the BsuRI recognition sequence: 5' dG--dG--dC--dC dC--dC--dG--dG 5'.Keywords
This publication has 7 references indexed in Scilit:
- Specificity ofHpaII andHaeII DNA methylasesNucleic Acids Research, 1977
- DNA methylation in adenovirus, adenovirus-transformed cells, and host cells.Proceedings of the National Academy of Sciences, 1976
- Restriction EndonucleaseCRC Critical Reviews in Biochemistry, 1976
- Restriction and modification in B. subtilisMolecular Genetics and Genomics, 1975
- Restriction and modification in B. subtilisMolecular Genetics and Genomics, 1975
- Genetic and transfection studies with B. subtilis phage SP 50Molecular Genetics and Genomics, 1970
- The frequencies of certain sequences of nucleotides in deoxyribonucleic acidBiochemical Journal, 1960