The Specificity of Translational Control Switched with Transfer RNA Identity Rules
- 21 February 1992
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 255 (5047), 994-996
- https://doi.org/10.1126/science.1372129
Abstract
The interaction of Escherichia coli threonyl-transfer RNA (tRNA) synthetase with the leader sequence of its own messenger RNA inhibits ribosome binding, resulting in negative translational feedback regulation. The leader sequence resembles the substrate (tRNA(Thr)) of the enzyme, and the nucleotides that mediate the correct recognition of the leader and the tRNA may be the same. A mutation suggested by tRNA identity rules that switches the resemblance of the leader sequence from tRNA(Thr) to tRNA(Met) causes the translation of the threonyl-tRNA synthetase messenger RNA to become regulated by methionyl-tRNA synthetase. This identity swap in the leader messenger RNA indicates that tRNA identity rules may be extended to interactions of synthetases with other RNAs.Keywords
This publication has 15 references indexed in Scilit:
- Escherichia coli threonyl-tRNA synthetase and tRNAThr modulate the binding of the ribosome to the translational initiation site of the ThrS mRNAJournal of Molecular Biology, 1990
- Genomic organization and physical mapping of the transfer RNA genes in Escherichia coli K12Journal of Molecular Biology, 1990
- Structure of E. coli Glutaminyl-tRNA Synthetase Complexed with tRNA Gln and ATP at 2.8 Å ResolutionScience, 1989
- tRNA IDENTITYAnnual Review of Biochemistry, 1989
- Messenger RNA structure and gene regulation at the translational level in Escherichia coli: the case of threonine:tRNAThr ligase.Proceedings of the National Academy of Sciences, 1988
- Genetic engineering of methionyl-tRNA synthetase: in vitro regeneration of an active synthetase by proteolytic cleavage of a methionyl-tRNA synthetase-β-galactosidase chimeric proteinBiochimie, 1988
- Differences between transfer RNA moleculesJournal of Molecular Biology, 1987
- RIBOSOMAL GENES IN ESCHERICHIA COLIAnnual Review of Genetics, 1986
- Genetic definition of the translational operator of the threonine-tRNA ligase gene in Escherichia coli.Proceedings of the National Academy of Sciences, 1986
- Autogenous control of Escherichia coli threonyl-tRNA synthetase expression in VivoJournal of Molecular Biology, 1985