The Mixed Agglutination Test in Studies on Human Transplantation

Abstract

Mixed agglutination with cell cultures was originally described by Högman¹and by Fagraeus and Espmark.²In this laboratory, application of this procedure for various research purposes^3-10was explored. One promising line seemed to be the detection of humoral antibodies accompanying homograft rejection in mice, rats, and rabbits.^7-10 In the present paper, preliminary studies on the demonstration of antibodies appearing as a result of homograft rejection in humans, as well as studies on human tissue typing will be reported. Materials and Methods Human Cell Lines.— HeLa,¹¹AV-3,¹²WI-38,¹³HEp-2,¹⁴D-6,¹⁵and KB¹⁶cells were cultured as described previously^3,4and studied when a whole monolayer was developed, usually after seven days of incubation. Primary cell cultureswere set up from human tonsils. The tissues were obtained from Children's Hospital, Buffalo, immediately after tonsillectomy. In preparing cell cultures, previously described methods^3,4