Adenosine stimulation of fluid transport across rabbit corneal endothelium
- 1 December 1977
- journal article
- research article
- Published by Springer Nature in The Journal of Membrane Biology
- Vol. 35 (1), 95-112
- https://doi.org/10.1007/bf01869942
Abstract
The rate of fluid transport across rabbit corneal endothelium has been measured with an automatic volumetric method. The present resolution of the procedure is 1–3 nanoliters, and intervals of measurement can be made as small as seconds. In the presence of glucose, oxidized glutathione (GSSG), and adenosine, the maximal rates were 6.2±1.0 μl/hr cm2, and 8.2±0.8 μl/hr cm2 if a large portion of the stroma was dissected away. In the presence of glucose and GSSG only, the rates were lower, namely 3.7±0.5 μl/hr cm2. The rates consistently increased or decreased when adenosine was added or deleted, respectively, during given experiments. The stimulation of fluid transport by adenosine was in the order of 40–50%. The results raise the possibility that this transport mechanism might be subject to metabolic control.This publication has 14 references indexed in Scilit:
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