Comparison of Low-Molecular-Weight Products Following Reaction of C3-C3b with C3b Inactivator and with Trypsin
- 30 September 1977
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 33 (4), 212-220
- https://doi.org/10.1111/j.1423-0410.1977.tb04466.x
Abstract
Substitution of trypsin for Conglutinogen-activating factor (KAF) in the procedure for cleaving C3d [d fragment of complement component 3] from C3-C3b substrate produced a relatively heterogeneous low-MW fraction (C3d-Tryp) which differed in a number of ways from the KAF-mediated cleavage product (C3d-KAF). The differences were demonstrable by agar and polyacrylamide gel electrophoresis, 125I-labeling, content of immunoreactive 125I-labeled C3d, inhibition of anti-complement antiglobulin reagents and rabbit immunization. By comparison with C3d-KAF, the C3d in C3d-Tryp was more heterogeneous and exhibited a faster electrophoretic mobility in agar at pH 8.6. By contrast to C3d-KAF, C3d-Tryp contained protein carrying C3c antigenic determinants.This publication has 13 references indexed in Scilit:
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