Comparative Study of Dehydration
- 1 January 1936
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 11 (1), 13-22
- https://doi.org/10.3109/10520293609111317
Abstract
The paraffin method has frequently been criticised because of its hardening and shrinking effect on tissue. The author believes this distortion is due to the dehydration and not to the immersion in melted paraffin. An experimentally controlled series of various tissues was dehydrated in different dehydrating reagents, dioxan, isobutyl alcohol, and ethyl alcohol with chloroform. Except for the dehydration, the tissues were treated identically. In every case, dioxan proved to be a better dehydrating reagent with less shrinkage and brittleness than any of the others. Ethyl alcohol with chloroform produced the greatest degree of distortion.Keywords
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