Association of Many Regions of the Bacillus subtilis Chromosome with the Cell Membrane
- 1 May 1973
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 114 (2), 571-6
- https://doi.org/10.1128/jb.114.2.571-576.1973
Abstract
Unsheared lysates of Bacillus subtilis 168T(-) containing uniformly labeled deoxyribonucleic acid (DNA) were exposed to varying doses of gamma rays to introduce double-strand scissions in the chromosome. From an estimate of the number-average molecular weight and the amount of DNA bound to membrane after irradiation, about 70 to 90 regions of the bacterial chromosome were detected in membrane fractions. Since this number was independent of the molecular weight of the DNA (i.e., the extent of fragmentation of the chromosome), it is thought to represent an upper limit in the number of membrane-binding sites per chromosome.Keywords
This publication has 18 references indexed in Scilit:
- DNA-membrane association and the repair of double breaks in X-irradiated Micrococcus radioduransBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- Competence for genetic transformation and the release of DNA from Bacillus subtilisBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- Evidence for stable attachment of DNA to membrane at the replication origin of Escherichia coliBiochemical and Biophysical Research Communications, 1970
- Spontaneous release of DNA in sequential genetic order by Bacillus subtilisJournal of Molecular Biology, 1969
- DNA Strand Breakage in Cells irradiated with X-raysNature, 1969
- Intracellular location and number of replicating parental DNA molecules of bacteriophages lambda and φX174Journal of Molecular Biology, 1969
- Shear Sensitivity of the E. coli Genome: Multiple Membrane Attachment Points of the E. coli DNACold Spring Harbor Symposia on Quantitative Biology, 1968
- Properties of the growing point region in the bacterial chromosomeBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967
- A cell-membrane bound fraction of bacterial DNABiochemical and Biophysical Research Communications, 1965
- Sedimentation Rate as a Measure of Molecular Weight of DNABiophysical Journal, 1963