Characterization of the functional gene and several processed pseudogenes in the human triosephosphate isomerase gene family.
Open Access
- 1 July 1985
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 5 (7), 1694-1706
- https://doi.org/10.1128/mcb.5.7.1694
Abstract
The functional gene and three intronless pseudogenes for human triosephosphate isomerase were isolated from a recombinant DNA library and characterized in detail. The functional gene spans 3.5 kilobase pairs and is split into seven exons. Its promoter contains putative TATA and CCAAT boxes and is extremely rich in G and C residues (76%). The pseudogenes share a high degree of homology with the functional gene but contain mutations that preclude the synthesis of an active triosephosphate isomerase enzyme. Sequence divergence calculations indicate that these pseudogenes arose approximately 18 million years ago. We present evidence that there is a single functional gene in the human triosephosphate isomerase gene family.This publication has 57 references indexed in Scilit:
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