Investigation of the Effect of Growth Environment on the Stability of Low-copy-number Plasmids in Escherichia coli.
- 1 July 1987
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 133 (7), 1881-1889
- https://doi.org/10.1099/00221287-133-7-1881
Abstract
The stability of a low-copy-number plasmid, pHSG415, in Escherichia coli, was investigated in batch and continuous culture. The plasmid was unstable in batch culture, but was significantly stabilized by growth in continuous culture with phosphate, nitrogen or potassium limitation. However, the plasmid was very unstable when grown in continuous culture with sulphate limitation. These results contrast with those obtained with multicopy plasmids such as pBR322, which is particularly unstable in carbon- or phosphate-limited continuous culture. The effect of growth rate on the stability of E. coli(pHSG415) grown in continuous culture with glucose limitation was also investigated. The plasmid was significantly more stable in cells grown at higher growth rates. The segregational instability (R) of the plasmid and the difference in growth rate between plasmid-free and plasmid-bearing cells (d.mu.) were calculated for each condition using the method of Cooper et al. (accompanying paper: Journal of General Microbiology 133. 1871-1880). It was found that the primary cause of the loss of pHSG415 from the cell population was the segregational instability of the plasmid.This publication has 14 references indexed in Scilit:
- Structural and functional analysis of the par region of the pSC101 plasmidCell, 1984
- Dual-origin plasmid vectors whose origin of replication is controlled by the coliphage lambda promoter pLGene, 1984
- Multimerization of high copy number plasmids causes instability: Cole 1 encodes a determinant essential for plasmid monomerization and stabilityCell, 1984
- Stability of a cloned gene in yeast grown in chemostat cultureMolecular Genetics and Genomics, 1983
- The use of a partition locus to increase stability of tryptophan-operon-bearing plasmids in Escherichia coliGene, 1983
- Specific-purpose plasmid cloning vectors I. Low copy number, temperature-sensitive, mobilization-defective pSC101-derived containment vectorsGene, 1981
- Maintenance and genetic stability of vector plasmids pBR322 and pBR325 in Escherichia coli K12 strains grown in a chemostatMolecular Genetics and Genomics, 1981
- Maintenance of some ColE1-type plasmids in chemostat cultureMolecular Genetics and Genomics, 1980
- Partitioning of bacterial plasmids during cell division: a cis-acting locus that accomplishes stable plasmid inheritanceCell, 1980
- Phenotypic Stability of trp Operon Recombinant Plasmids in Escherichia coliMicrobiology, 1980