Patterns of Cytopathology and Lysosomal Enzyme Release in Poliovirus-infected HEp-2 Cells Treated With Either 2-( -Hydroxybenzyl)-Benzimidazole or Guanidine HCl

Abstract

Summary The viral inhibitors guanidine hydrochloride and 2-(α-hydroxybenzyl)-benzimidazole (HBB) were used to study the relationship between lysosomal enzyme release and the development of poliovirus-induced cytopathic effect (CPE) in HEp-2 cells. Lysosomal enzyme release, the development of CPE and virus replication were inhibited or delayed if these antiviral agents were added up to 2 to 3 hr after infection. When added later the agents were no longer effective. It is suggested that a virus-induced protein produced during 3 hr after infection is responsible for lysosomal enzyme release.