Lactate biosensor based on a lactate dehydrogenase/nicotinamide adenine dinucleotide biocomposite
- 1 October 1994
- journal article
- research article
- Published by Wiley in Electroanalysis
- Vol. 6 (10), 850-854
- https://doi.org/10.1002/elan.1140061007
Abstract
A new biocomposite, fabricated by incorporating lactate dehydrogenase (LDH) and nicotinamide adenine dinucleotide (NAD+) within a graphite‐epoxy matrix, is used as a polishable (renewable) and robust biosensor for lactate. Highly sensitive and rapid amperometric biosensing is accomplished at relatively low potentials. The regeneration capability of the biocomposite probe circumvents problems of surface fouling inherent to the detection of NADH. Flow injection detection of lactate, with a sample throughput of 60/h, detection limit of 5 × 10−4 M, precision of 2.5% (RSD for n = 40), and linearity up to 2 × 10−2 M, is illustrated.This publication has 18 references indexed in Scilit:
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