Preparation of Protoplasts and Whole Cell Ghosts from Mycobacterium smegmatis
- 1 December 1979
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 115 (2), 517-521
- https://doi.org/10.1099/00221287-115-2-517
Abstract
Cell wall-deficient forms of M. smegmatis were produced in growth medium containing D-cycloserine and horse serum. These cells were transformed into protoplasts with EDTA and lysozyme. Subsequent lysis by nucleases followed by osmotic shock produced membrane vesicles (whole cell ghosts).This publication has 8 references indexed in Scilit:
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