A DNA sequence upstream of the tet(O) gene is required for full expression of tetracycline resistance
Open Access
- 1 October 1991
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 35 (10), 2020-2025
- https://doi.org/10.1128/aac.35.10.2020
Abstract
The DNA sequences upstream of the tet(O) and tet(M) open reading frames (ORFs) (ca. 300 bp) were found to share a higher degree of homology than those of the tet(O) and tet(M) ORFs themselves. A transcription initiation site for tet(O) was located by primer extension analysis. Campylobacter coli was found to use a promoter sequence different from that used by Escherichia coli. The sequence upstream of tet(O) was shown to be required in cis for high-level resistance to tetracycline.Keywords
This publication has 22 references indexed in Scilit:
- Tetracycline resistance heterogeneity in Enterococcus faeciumAntimicrobial Agents and Chemotherapy, 1991
- Cloning and nucleotide sequence of a chromosomally encoded tetracycline resistance determinant, tetA(M), from a pathogenic, methicillin-resistant strain of Staphylococcus aureusAntimicrobial Agents and Chemotherapy, 1990
- Chloramphenicol resistance in Campylobacter coli: nucleotide sequence, expression, and cloning vector constructionGene, 1990
- Molecular studies on the mechanism of tetracycline resistance mediated by Tet(O)Antimicrobial Agents and Chemotherapy, 1990
- Nucleotide sequence analysis and expression of a tetracycline-resistance gene from Campylobacter jejuniGene, 1988
- [1] Production of single-stranded plasmid DNAMethods in Enzymology, 1987
- A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.Proceedings of the National Academy of Sciences, 1985
- Rapid and efficient site-specific mutagenesis without phenotypic selection.Proceedings of the National Academy of Sciences, 1985
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- REGULATORY SEQUENCES INVOLVED IN THE PROMOTION AND TERMINATION OF RNA TRANSCRIPTIONAnnual Review of Genetics, 1979