Protein kinase C subtypes in human neutrophils
- 1 January 1991
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 51 (3), 299-302
- https://doi.org/10.3109/00365519109091618
Abstract
Protein kinase C from rat brain and human neutrophils was chromatographically separated on a hydroxylapatite column connected to a high-performance liquid chromatography (HPLC) system. Protein kinase C from rat brain was separated into three peaks (types I, II, and III). In contrast, only two types were obtained from human neutrophils matching type II and type III in brain. In cell membranes from unstimulated human neutrophils type III was predominant, but mainly type II was translocated to the membranes upon stimulation with phorbolmyristatacetate (PMA). Both types were equivalently activated by phosphatidylserine, diolein, and magnium and presented identical Km for ATP.Keywords
This publication has 8 references indexed in Scilit:
- A time-course study on superoxide generation and protein kinase C activation in human neutrophilsFEBS Letters, 1988
- The heterogeneity of protein kinase C in various rat tissuesBiochemical and Biophysical Research Communications, 1988
- Identification of three additional members of rat protein kinase C family: gd‐, ϵ‐ and ξ‐subspeciesFEBS Letters, 1987
- Protein kinase C and the activation of the human neutrophil NADPH- oxidaseBlood, 1987
- Membrane-associated protein kinases in phorbol ester-activated human polymorphonuclear leukocytesBiochimica et Biophysica Acta (BBA) - General Subjects, 1986
- The Complete Primary Structure of Protein Kinase C—the Major Phorbol Ester ReceptorScience, 1986
- Purification and properties of protein kinase C from bovine polymorphonuclear leucocytesBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1986
- Production of Superoxide by Phagocytic Leukocytes: A Paradigm for Stimulus-Response PhenomenaCurrent Topics in Cellular Regulation, 1986