CIRCULATING ANTIBODIES TO THE SURFACE-ANTIGENS ON COLON EPITHELIAL-CELLS IN ULCERATIVE-COLITIS

  • 1 January 1983
    • journal article
    • research article
    • Vol. 54 (1), 163-168
Abstract
The fluorescence activated cell sorter (FACS) was used for detecting circulating antibodies to the surface antigens on isolated [rat] colon epithelial cells (anti-colon antibodies) by indirect immunofluorescence. Anti-colon antibodies were found in the serum of 30 of 41 (73%) patients with ulcerative colitis. This incidence is much higher than one established in earlier reports by application of indirect immunofluoresence to colon tissue using the fluorescence microscope. FACS analysis is apparently very useful for detecting antibodies to colon specific antigen.