MYCOBACTERIAL CELL WALLS I
- 1 January 1963
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 85 (1), 16-23
- https://doi.org/10.1128/jb.85.1.16-23.1963
Abstract
Takeya, Kenji (Kyushu University, Fukuoka, Japan) and Kazuhito Hisatsune. Mycobacterial cell walls. I. Methods of preparation and treatment with various chemicals. J. Bacteriol. 85:16–23. 1963.—Several methods of preparation of mycobacterial cell walls were examined, and the grinding method with glass powder, using Dry Ice, was found to give fairly good cell-wall preparations. “Paired fibrous structures” were clearly seen on the purified cell wall. The appearance of the cell wall as revealed by the electron microscope was not altered by digestion with trypsin, pronase, or pronase in 5% alcoholic solution, nor by treatment with 95% alcohol, acetone-alcohol mixture, or ether-alcohol mixture. By treatment with alcoholic KOH solution, the fibrous structure was removed. The remaining thin layer of the cell wall was tentatively designated the “basal layer” of the mycobacterial cell wall. The fibers appeared also to be removed by chloroform treatment. Nagarse digestion seemed to solubilize some constituents of the cell wall. The cell wall lost its shape and rigidity after lysozyme digestion. ImagesKeywords
This publication has 11 references indexed in Scilit:
- MYCOBACTERIAL CELL WALLS IIJournal of Bacteriology, 1963
- Electron Microscope Observations on the Surface Structures of Streptomyces violaceoruberJournal of General Microbiology, 1961
- FURTHER STUDIES ON THE PAIRED FIBROUS STRUCTURE OF MYCOBACTERIAL CELL WALLThe Journal of cell biology, 1961
- IMMUNOGENICITY OF INTRACELLULAR PARTICLES AND CELL WALLS FROM MYCOBACTERIUM TUBERCULOSISJournal of Bacteriology, 1960
- ALLERGENICITY OF INTRACELLULAR PARTICLES, CELL WALLS, AND CYTOPLASMIC FLUID FROM MYCOBACTERIUM TUBERCULOSISJournal of Bacteriology, 1960
- The Rigid Layer of the Cell Wall of Escherichia coli Strain BJournal of General Microbiology, 1960
- Paired fibrous structure of mycobacteriaBiochimica et Biophysica Acta, 1958
- Immunologic Significance of the Cell Wall of MycobacteriaExperimental Biology and Medicine, 1958
- Studies on the Cell-Wall Composition and Taxonomy of Actinomycetales and Related GroupsJournal of General Microbiology, 1958
- USE OF GLASS BEADS FOR THE MECHANICAL RUPTURE OF MICROORGANISMS IN CONCENTRATED SUSPENSIONSJournal of Bacteriology, 1954