Methyl 13-D- and 13-L-hydroxydocosanoates have been synthesized from 9-D- and 9-L-hydroxy-octadecanoic acids. Comparison of these esters with the methyl ester of 13-hydroxydocosanoic acid, produced by Candida bogoriensis, shows that the natural product has the L-configuration. The known 2-D-octadecanol has been prepared from 17-hydroxyoctadecanoic acid, produced by Torulopsis apicola, showing that this acid also has the L-configuration. Most naturally occurring long-chain hydroxy acids, however, have the D-configuration.