Effect of Hoechst 33258 on Chinese hamster chromosomes
- 1 March 1976
- journal article
- research article
- Published by Springer Nature in Chromosoma
- Vol. 56 (1), 41-46
- https://doi.org/10.1007/bf00293728
Abstract
Cells of the Chinese hamster strain C-125 were treated for different time intervals with H 33258, a bibenzimidazole derivative. The same compound was used to stain fixed cells of the same strain. — H 33258 induced in cells in culture specific areas of reduced spiralization on the metaphase chromosomes of some cells. These probably correspond to DNA segments rich in A-T bases interspersed along the chromosomes. Probably H 33258 acts during S period of cell cycle. — The banding obtained by staining with H 33258 is similar to that induced by quinacrine dihydrochloride but shows a better resolution.Keywords
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