Elimination of matrix effects in the determination of bisphenol A in milk by solid-phase microextraction–high-performance liquid chromatography
- 17 May 2008
- journal article
- research article
- Published by Taylor & Francis in Food Additives & Contaminants: Part A
- Vol. 25 (6), 772-778
- https://doi.org/10.1080/02652030701713921
Abstract
Solid-phase microextraction coupled to high-performance liquid chromatography (SPME–HPLC) with fluorescence detection was employed to determine bisphenol A (BPA) in milk samples. The potential influence of the milk matrix on the determination of BPA by SPME–HPLC were investigated. Optimal conditions to eliminate any matrix effects were as follows: milk samples were deproteinized with trichloroacetic acid, diluted 20-fold with BPA-free Ultrapure water, dissolved in methanol, the precipitated protein was filtered out, rinsed with methanol and evaporated to remove the methanol. Then, a 40.0-ml solution was used for SPME extraction and HPLC analysis. Satisfactory recoveries (milk: 93.1–101%; soybean milk: 93.9–102%) were achieved. The proposed method was successfully applied to real samples, BPA being detected within the range 1.6–2.6 ng ml−1 in four brands of commercial milk but not in soybean milk.Keywords
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