EVALUATION OF POLIOMYELITIS ANTISERA BY PLAQUE NEUTRALIZATION TESTS

Abstract

After the reproducibility of poliovirus plaque titrations was estimated, the course of specific neutralization of virus by antiserum at 37 °C was investigated and was found to be logarithmic with respect to time and dilution of serum. A method was adopted for titrating poliomyelitis plaque neutralizing antibodies under defined test conditions. The titers obtained by this method were much more reproducible than those found by other methods in current use, but bore fairly constant relationships to the average values found in larger series of titrations by the latter methods.