A temperature-jump study of the reaction between azurin and cytochrome c oxidase from Pseudomonas aeruginosa
- 1 October 1975
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 151 (1), 185-188
- https://doi.org/10.1042/bj1510185
Abstract
The electron-transfer reaction between azurin and the cytochrome oxidase from Pseudomonas aeruginosa was investigated by temperature-jump relaxation in the absence of O2 and in the presence of CO. The results show that: (i) reduced azurin exists in two forms in equilibrium, only one of which is capable of exchanging electrons with the Pseudomonas cytochrome oxidase, in agreement with M. T. Wilson, C. Greenwood, M. Brunori & E. Antonini (1975) (Biochem. J. 145, 449-457); (ii) the electron transfer between azurin and Pseudomonas cytochrome oxidase occurs within a molecular complex of the two proteins; this internal transfer becomes rate-limiting at high reagent concentrations.Keywords
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