Batch Injection Analysis with Thermistor Sensing Devices

Abstract

Batch injection analysis (BIA) is a new non-flow technique involving the injection of microliter samples toward a nearby detector, immersed in a large-volume blank solution. This paper describes the characteristics and advantages of employing thermal sensing devices as detectors for BIA. Similar to analogous flow injection measurements, batch injection thermal analysis offers high speed, reproducibility and simplicity, while eliminating the need for pumps, valves and associated tubing. There is no observable carryover and the precision is typically 2% (RSD). The batch injection thermal analysis is illustrated for enzymatic reactions, as well as redox and acid-base reactions.