Effect of 22,25-Diazacholestanol on Synthesis of Cholesterol by Rat Liver Homogenates

Abstract
Summary In rat liver homogenates 22,25-diazacholestanol blocked to the same extent the incorporation into cholesterol of 2-C14-acetate, 2-C14-mevalonate and of 26,27-C14-desmosterol. Thus, direct evidence is provided for a triparanol-like interference with hepatic cholesterogenesis in vitro. This is in agreement with the finding of appreciable desmosterol levels in humans and rats treated with 22,25-diazacholestanol per os.