Genome-wide gene regulation of biosynthesis and energy generation by a novel transcriptional repressor in Geobacter species
Open Access
- 25 November 2009
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 38 (3), 810-821
- https://doi.org/10.1093/nar/gkp1085
Abstract
Geobacter species play important roles in bioremediation of contaminated environments and in electricity production from waste organic matter in microbial fuel cells. To better understand physiology of Geobacter species, expression and function of citrate synthase, a key enzyme in the TCA cycle that is important for organic acid oxidation in Geobacter species, was investigated. Geobacter sulfurreducens did not require citrate synthase for growth with hydrogen as the electron donor and fumarate as the electron acceptor. Expression of the citrate synthase gene, gltA, was repressed by a transcription factor under this growth condition. Functional and comparative genomics approaches, coupled with genetic and biochemical assays, identified a novel transcription factor termed HgtR that acts as a repressor for gltA. Further analysis revealed that HgtR is a global regulator for genes involved in biosynthesis and energy generation in Geobacter species. The hgtR gene was essential for growth with hydrogen, during which hgtR expression was induced. These findings provide important new insights into the mechanisms by which Geobacter species regulate their central metabolism under different environmental conditions.Keywords
This publication has 69 references indexed in Scilit:
- Selection of a variant of Geobacter sulfurreducens with enhanced capacity for current production in microbial fuel cellsBiosensors and Bioelectronics, 2009
- Genome-wide analysis of the RpoN regulon in Geobacter sulfurreducensBMC Genomics, 2009
- Power output and columbic efficiencies from biofilms of Geobacter sulfurreducens comparable to mixed community microbial fuel cellsEnvironmental Microbiology, 2008
- Genes for two multicopper proteins required for Fe(III) oxide reduction in Geobacter sulfurreducens have different expression patterns both in the subsurface and on energy-harvesting electrodesMicrobiology, 2008
- Computational and Experimental Analysis of Redundancy in the Central Metabolism of Geobacter sulfurreducensPLoS Computational Biology, 2008
- Computational prediction of RpoS and RpoD regulatory sites in Geobacter sulfurreducens using sequence and gene expression informationGene, 2006
- Identification of a Gene Involved in Polysaccharide Export as a Transcription Target of FruA, an Essential Factor for Myxococcus xanthus DevelopmentPublished by Elsevier ,2005
- CcpC, a novel regulator of the LysR family required for glucose repression of the citB gene in Bacillus subtilisJournal of Molecular Biology, 2000
- Citrate synthase and 2-methylcitrate synthase: structural, functional and evolutionary relationshipsMicrobiology, 1998
- Studies on transformation of Escherichia coli with plasmidsJournal of Molecular Biology, 1983