Effect of unmodified triple helix‐forming oligodeoxyribonucleotide targeted to human multidrug‐resistance gene mdr1 in MDR cancer cells
- 3 October 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 352 (3), 380-384
- https://doi.org/10.1016/0014-5793(94)00995-3
Abstract
The human mdr1 gene encodes a transmembrane glycoprotein the over-expression of which is associated with development of multidrug resistance in human tumor cells. A negative modulation of human mdr1 has been attempted via a 27-mer unmodified triple helix-forming oligonucleotide, named 1D, targeted to a homopurine sequence in the coding region of the gene. By administering 10 μM of 1D we could find a significant reduction in MDR1 mRNA levels in the human drug-resistant cell line CEM-VLB 100. This effect appears to be specific and due to a transient block of RNA polymerase mediated by triple helix formationKeywords
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