Anin VitroClonogenic Assay to Assess Radiation Damage in Rat CNS Glial Progenitor Cells
- 1 January 1990
- journal article
- other
- Published by Taylor & Francis in International Journal of Radiation Biology
- Vol. 58 (5), 835-844
- https://doi.org/10.1080/09553009014552211
Abstract
Normal glial progenitor cells can be isolated from the rat central nervous system (CNS) and cultured in vitro on a monolayer of type-1 astrocytes. These monolayers are able to support and stimulate explanted glial progenitor cells to proliferate. Employing these in vitro interactions of specific glial cell types, an in vivo-in vitro clonogenic assay has been developed. This method offers the possibility to study the intrinsic radiosensitivity, repair and regeneration of glial progenitor cells after in vitro or in vivo irradiation.Keywords
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