Determination and Quantitation of Bendroflumethiazide and Its Degradation Products Using HPLC

Abstract

A method for the determination and quantitation of bendroflumethiazide (BFMT) and its degradation products hydroflumethiazide (HFMT) and 5-trifluoromethyl-2, 4-disphamoylaniline (TFSA) using RP-HPLC is described. Good reproducibility and accuracy of the assay can be achieved without the use of a buffer solution in the mobile phase. A pH of 2.0 of the solvent and the use of dark flasks will be important to give a good stability of the samples during automatic injection. The limits of detection for BFMT, HFMT and TFSA were respectively 1.3, 0.8 and 0.7 μ/ml (α = 0.05).