CULTURE AND HETEROTROPHY OF THE FRESHWATER DINOFLAGELLATE, PERIDINIUM CINCTUM FA. OVOPLANUM LINDEMAN1

Abstract

-A defined minimal medium was developed for an axenic strain of Peridinium (Indiana Culture No. LB 1336). Thiamine, biotin, and vitamin B12 did not stimulate growth. Of 15 organic C sources tried in light, fructose, galactose, glucose, malate, malonate, and pyruvate enhanced growth but propionate retarded growth. In dark-grown cultures only media with succinate permitted growth above the survival level. Stimulation of growth by organic C sources was markedly pH dependent.