Branching of Central Neurons: Intracellular Cobalt Injection for Light and Electron Microscopy
- 28 April 1972
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 176 (4033), 412-414
- https://doi.org/10.1126/science.176.4033.412
Abstract
Cobalt chloride can be injected into an identified nerve cell body in an insect ganglion and reacted with ammonium sulfide to stain the soma and its branches with a black precipitate. The stained cell body and its branches throughout the neuropil are visible in both the light and electron microscope. In whole mount preparations, the resolution of neurites within the neuropil is of a quality that permits the comparison of branching patterns between cells and during various functional states.Keywords
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