Aminofluorenols as Urinary Metabolites of N-2-Fluorenylacetamide

Abstract

The isomeric hydroxylated derivatives of 2-fluorenamine, i.e., the 2-amino-1-, -3-, -5-, -7-, and -8-fluorenols, were found in small amounts as free compounds and as glucuronic acid conjugates in the urine of rats given N-2-fluorenylacetamide. The presence of the metabolites was established by specially designed solvent partition steps followed by chromatography and specific isotope-dilution procedures. The aminofluorenols could be the result of the hydroxylation of 2-fluorenamine or the deacetylation of the corresponding N-(hydroxy-2-fluorenyl) acetamides which are known metabolites of N-2-fluorenylacetamide.